Review



phycoerythrin pe conjugated rat anti mouse cxcr4 antibody  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems phycoerythrin pe conjugated rat anti mouse cxcr4 antibody
    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of <t>CXCR4+</t> cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.
    Phycoerythrin Pe Conjugated Rat Anti Mouse Cxcr4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe conjugated rat anti mouse cxcr4 antibody/product/R&D Systems
    Average 94 stars, based on 29 article reviews
    phycoerythrin pe conjugated rat anti mouse cxcr4 antibody - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "Characterization of the mesendoderm progenitors in the gastrulating mouse embryo"

    Article Title: Characterization of the mesendoderm progenitors in the gastrulating mouse embryo

    Journal: bioRxiv

    doi: 10.1101/2024.04.28.591221

    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of CXCR4+ cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.
    Figure Legend Snippet: A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of CXCR4+ cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.

    Techniques Used: Expressing, RNA Sequencing Assay, Activity Assay, Comparison, Flow Cytometry



    Similar Products

    phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265  (Bioss)
    93
    Bioss phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265
    Phycoerythrin Pe Conjugated Mouse Anti Rat Cxcr4 Antibodies 265, supplied by Bioss, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265/product/Bioss
    Average 93 stars, based on 1 article reviews
    phycoerythrin pe conjugated mouse anti rat cxcr4 antibodies 265 - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    phycoerythrin pe conjugated rat anti mouse cxcr4 antibody  (R&D Systems)
    94
    R&D Systems phycoerythrin pe conjugated rat anti mouse cxcr4 antibody
    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of <t>CXCR4+</t> cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.
    Phycoerythrin Pe Conjugated Rat Anti Mouse Cxcr4 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin pe conjugated rat anti mouse cxcr4 antibody/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    phycoerythrin pe conjugated rat anti mouse cxcr4 antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    phycoerythrin (pe)-conjugated rat anti-mouse cxcr4 antibody  (Becton Dickinson)
    90
    Becton Dickinson phycoerythrin (pe)-conjugated rat anti-mouse cxcr4 antibody
    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of <t>CXCR4+</t> cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.
    Phycoerythrin (Pe) Conjugated Rat Anti Mouse Cxcr4 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin (pe)-conjugated rat anti-mouse cxcr4 antibody/product/Becton Dickinson
    Average 90 stars, based on 1 article reviews
    phycoerythrin (pe)-conjugated rat anti-mouse cxcr4 antibody - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    phycoerythrin (pe)-conjugated rat anti-mouse antibody against cxcr4 2b11  (Thermo Fisher)
    90
    Thermo Fisher phycoerythrin (pe)-conjugated rat anti-mouse antibody against cxcr4 2b11
    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of <t>CXCR4+</t> cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.
    Phycoerythrin (Pe) Conjugated Rat Anti Mouse Antibody Against Cxcr4 2b11, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phycoerythrin (pe)-conjugated rat anti-mouse antibody against cxcr4 2b11/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    phycoerythrin (pe)-conjugated rat anti-mouse antibody against cxcr4 2b11 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of CXCR4+ cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.

    Journal: bioRxiv

    Article Title: Characterization of the mesendoderm progenitors in the gastrulating mouse embryo

    doi: 10.1101/2024.04.28.591221

    Figure Lengend Snippet: A. EpiSC lines of Mixl1WT and Mixl1KO genotypes, and harboring FLAG-Mixl1 in HPRT locus that can be activated by induction with Doxycycline. Gene editing of Mixl1 locus in Mixl1 KO and Mixl1 Dox inducible cell line. B. The expression of Mixl1 in the cell lines induced by Doxycycline treatment on Day 0, 1, 2 and 3 (D0DOX, D1DOX, D2 DOX and D3 DOX respectively).The panel highlights (grey) the time when expression peaks for individual Dox induction timings. C. Principal component analysis of gene expression profile assayed by RNA sequencing of the samples-D1Dox (Day 2), NoDox(Day 2), D0Dox (Day4), NoDox (Day 4). D. Heat map presentation of Mixl1 responsive genes upregulated with Day 1 (early) and Day 3 (late) induction of Mixl1 activity in comparison to NoDox treatment. Gene Ontology enrichment analysis of the upregulated genes with Day 1 versus Day 3. E. Flow cytometry analysis of CXCR4+ cells in the differentiating EpiSCs with different timing of Mixl1 induction (N=3). The left panel is all CXCR4+ cells as a percentage of total cells. The right panel includes cells that were gated as highly positive for CXCR4. F. Expression of Sox17 transcript in MixL1WT, MixL1KO or MixL1Dox mouse EpiSCs with either No Dox treatment or MixL1 induction via Doxycycline at Day 0 (D0Dox), 1 (D1Dox), 2 (D2Dox) and 3 (D3Dox) of differentiation.

    Article Snippet: CXCR4 (CD184) staining for endoderm propensity quantification was performed using phycoerythrin (PE)-conjugated rat anti-mouse CXCR4 antibody (R&D Systems) in Dulbecco’s phosphate-buffered saline with 10% Fetal Calf Serum (Flow cytometry buffer) for 45 minutes at room temperature and washed thrice in Flow cytometry buffer.

    Techniques: Expressing, RNA Sequencing Assay, Activity Assay, Comparison, Flow Cytometry